In our previous videos we introduced the CRISPR Cas9 system briefly (http://youtu.be/1aJxXWkE3Ek) and presented the different tools and methods available for their expression in living systems (http://youtu.be/INC_kdr7I34). Here we will go over the design criteria of the gRNA and introduce you to the online programs that simplify this procedure. We invite you to watch our previous videos first before continuing with this one.
For more information on how to design gRNA for CRISPR Cas9 systems, please visit:
➜ Knowledge Base: https://info.abmgood.com/crispr-cas9-sgrna-design
Chop Chop Harvard: https://chopchop.rc.fas.harvard.edu/
*Please note: There is an error at ~1:12. Cas9 has an HNH and an RuvC1-like nuclease domain (in the video, it was mislabeled as «NHN»).
Want more tips, tricks, guides, and protocols on how to knockout a gene using CRISPR? Join our free, 4-week #CRISPRCrashCourse: http://info.abmgood.com/crispr-crash-course
For more resources on CRISPR, visit:
➜ https://info.abmgood.com/crispr-cas9
*Spanish subtitles are also available
Watch the other videos in this series on CRISPR:
➜ Introduction: https://youtu.be/1aJxXWkE3Ek
➜ Methods and Tools: https://youtu.be/INC_kdr7I34
➜ CRISPR Playlist: https://youtu.be/1aJxXWkE3Ek?list=PLTt9kKfqE_0Ei8_rQsrfm01-zQtABTn0Z
Check out our other video series:
➜ Adeno Associated Virus: https://youtu.be/hYHbfQe5h-Q?list=PLTt9kKfqE_0HfXQMX9RPgbcmSWMqVtlBf
➜ Next Generation Sequencing: https://youtu.be/jFCD8Q6qSTM?list=PLTt9kKfqE_0Gem8hIcJEn7YcesuuKdt_n
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